In this Transformation of E-Coli Kit, students will explore the biological process of bacterial transformation using E-Coli and green fluorescent protein plasmid DNA.

At the end of the activity, students will have experience observing and analysing acquired traits (ampicillin resistance and fluorescence) as exhibited by transformed bacterial cells.

• Group Size: for 10 lab groups
• Storage: some components require refrigerator and freezer storage upon receipt
• Time Required: Set-up - 50 minutes, Incubation - overnight, Transformation - 15 minutes

The kit includes:

• Full instructions
• BactoBeads™ E.coli GFP Host
• Supercoiled pFluoroGreen™ plasmid DNA
• Ampicillin
• IPTG
• Calcium Chloride
• Growth Additive
• ReadyPour™ Luria Broth Agar (sterile)
• Luria Broth Medium for Recovery (sterile)
• Petri Plates (small & large)
• Plastic microtipped transfer pipettes
• Wrapped 10ml pipette (sterile)
• Toothpicks (sterile)
• Inoculating loops (sterile)
• Microcentrifuge tubes

Additional required equipment & materials (not supplied with the kit):

• Automatic micropipette (5-50µl)
• 2 x Water Bath (B8R06048)
• Thermometer (B8R05775)
• Incubator (B8A64397)
• Pipette Filler (B8A78062)
• Ice
• Marker Pens
• Bunsen Burner
• Microwave or Hot Plate
• Hot gloves
• Long wave UV light